8/31 11:00 - 1:00, Hewlett 200
Single Molecule Biology Meets Systems Biology
Professor of Chemistry and Chemical Biology, Harvard University.
Our group studied gene expression in living bacterial cells with single-molecule sensitivity with millisecond time resolution and nm spatial precision. We reported the first movie of protein production, one molecule at a time, binding and unbinding kinetics of transcription factors on DNA, and their single-molecule events that change the cell's phenotype. We recently conducted system-wide studies of transcriptome and proteome with single-molecule sensitivity in a single cell. We are investigating the phenomenon of persisters, abnormal and rare bacterial cells that have the same genes as normal cells, but are phenotypically drug tolerant, hoping to provide clues for developing tuberculosis drugs.
Xiaoliang Sunney Xie is the Mallinckrodt Professor of Chemistry and Chemical Biology at Harvard University. Xie was among the first to conduct fluorescence studies of single molecules at room temperature in early 1990s. His group has since contributed to the emergence of the now vital field of single-molecule science and its application to biology. His work focuses on single-molecule enzymology and biosphysics, and gene expression in living cells. Xie's team also has pioneered coherent Raman scattering microscopy, a highly sensitive imaging technique for living cells and organisms, which does not rely on fluorophores, but on vibrational contrast with molecular selectivity.