8/31 11:00 - 1:00, Annenberg Auditorium
Developing a single cell map of immune cell biology and dysfunction
Professor of Microbiology & Immunology, Stanford University.
Intracellular assays of signaling systems have been limited by an inability to correlate functional subsets of cells in complex populations based on active kinase states or other nodal signaling junctions. Such correlations could be important to distinguish changes in signaling status that arise in rare cell subsets during functional activation or in disease manifestation. We have demonstrated the ability to simultaneously detect activated kinases and phospho-proteins in simultaneous pathways in subpopulations of complex cell populations by multi-parameter flow cytometric analysis. We have applied this technology to the study of normal human cell populations, immune and auto-immune models, as well as human disease states including Acute Myelogenous Leukemia, and Follicular Lymphoma, Rheumatoid Arthritis and Lupus, among others. I will discuss our efforts in the development of a single cell map of immune cell biology and dysfunction, and finally our steps towards development of ultra-high parameter analysis of kinases states at the single cell level using mass spectrometry flow cytometry hybrid devices.
Dr. Nolan's laboratory at the Stanford University School of Medicine focuses on the analysis of biological events at the single cell level using novel genetic and FACS-based approaches at the intersection of immunology, autoimmunity, biochemistry, and cancer. The laboratory studies phospho-protein immune cell and cancer signaling, and other metabolic parameters by analysis of biochemical functions at the single cell level in primary cell populations. This includes interrogation of cancer (Cell, 2004) and immune signaling networks in complex cell populations (Science, 2005), and using multiparameter data to stratify signaling maps from patient samples, (Cancer Cell, 2008). Other major interest areas of the laboratory include mapping of signaling networks within complex populations of immune cells, developing systems biology approaches to develop an atlas of immune cell differentiation, the development of mechanism-based diagnostics for use in clinical trial studies.