Plenary 5.2

9/2 8:00 - 10:30, Memorial Auditorium

Post-translational Modifications in stress-induced Signaling Pathway

Kong-Joo Lee

Professor, Center for Cell Signaling amp;& Drug Discovery Research, College of Pharmacy & Division of Life & Pharmaceutical Sciences, Ewha Womans University, Seoul, Korea 120-750.

We are interested in understanding the regulation mechanism of proteins in stress-induced signaling pathways. Post-translational modifications (PTMs) of proteins play key roles in regulation of various signaling processes. Employing proteomic tool, we examined the PTMs, however, comprehensive identification of PTMs remains a highly challenging problem in proteomics, due to the dynamic complexities in vivo and low abundance of PTMs. We describe a new PTM-specific strategy to identify the comprehensive modifications. This strategy yields a rapid and efficient finding of multiple and comprehensive PTMs occurred in signaling pathways in vivo in >91% peptide coverage. In this talk, I will discuss in vivo PTMs which are more complicate and heterogeneous than previously reported and the results were further analyzed with dynamic modeling.

Kong-Joo LeeKong-Joo Lee received her Ph.D in 1986 from the Department of Chemistry at the Stanford University, USA. She worked as a post-doctoral fellow in Cancer Biology Program at Stanford Medical School, 1987-88, returned to Korea and worked as senior scientists in Korea Research Institute of Standards and Sciences (KRISS) 1989-1994, then as a professor in Ewha Womans University, College of Pharmacy, 1994-present. Now she is a director of National Core Research Center, “Center for Cell Signaling and Drug Discovery Research”, which is a program grant funded by KOSEF. Her lab combines biological, proteomic and informatic tools to understand signaling processes in stress- and metastasis-related, especially to understand the relationship between protein modifications and cellular processes.