Plenary Session 5 – Signaling Networks
9/2 8:00 - 10:30, Memorial Auditorium
Basal Dynamics of p53 in Single Cells
Our lab is interested in understanding how the dynamic behavior of biological signals is controlled and how these dynamics affect cellular responses. We focus on the signaling pathway of the tumor suppressor protein p53, which is the protein most frequently inactivated in human cancer. Our single cell imaging studies showed that DNA damage initiates a series of p53 pulses that vary in number from cell to cell. We have recently discovered that p53 shows spontaneous pulses even in the absence of external damage. In this talk I will discuss the structure of the network that shapes p53's dynamical behavior in response to extrinsic and intrinsic damage and will describe possible functions of these dynamics.
Post-translational Modifications in stress-induced Signaling Pathway
Ewha Womans University, Seoul, Korea 120-750.
We are interested in understanding the regulation mechanism of proteins in stress-induced signaling pathways. Post-translational modifications (PTMs) of proteins play key roles in regulation of various signaling processes. Employing proteomic tool, we examined the PTMs, however, comprehensive identification of PTMs remains a highly challenging problem in proteomics, due to the dynamic complexities in vivo and low abundance of PTMs. We describe a new PTM-specific strategy to identify the comprehensive modifications. This strategy yields a rapid and efficient finding of multiple and comprehensive PTMs occurred in signaling pathways in vivo in >91% peptide coverage. In this talk, I will discuss in vivo PTMs which are more complicate and heterogeneous than previously reported and the results were further analyzed with dynamic modeling.
University of California, San Francisco.
Peter K. Sorger